I am new to Linux and need some information. I have download the latest version of Ubuntu 64-bit from website. I want to run it in Virtual Machine, is this work i mean Virtual Machine run the ISO file or is there other file for Virtual Machine, because once i used the OSGEO live and it's completely different.
What is your objective ? It is not clear to me. Anyway if you want to install Ubuntu 64 bit OS in your VM with the ISO file, this is doable. You have to mount the iso file in the virtual CD drive and start the VM. What is the hypervisor you are using? I mean, is it VirtualBox, KVM or something else ? If you want to know something else, just let me know.
The ISO file is sufficient for what I believe you are asking. If you simply want to run Ubuntu in a VM you are on the right path and can use the ISO you have downloaded. What I have done in the past is created a folder in my home directory and named it "ISO Images" and then moved the ISO from "Downloads" folder to the newly created "ISO Images" folder. I have then opened the VM and during the set-up process, pointed the VM at the new folder and the desired iso image.
Hope this helps and good luck!