I am trying to set up a Ubuntu One account on my wife's laptop. When we put her email address in, it will not accept it.
I already have an account on my laptop and I can't remember having this problem when I set it up.
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Try logging in to Ubuntu One in your browser and check that the email address is the same as you are typing it in the Ubuntu One desktop application.
If you get any more errors post a screenshot or type the exact error, it will help us diagnose the problem faster.