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This question already has an answer here:

I want to modify wineprefix installed with playonlinux using Winetricks.

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marked as duplicate by Luis Alvarado Jul 24 '13 at 0:59

This question has been asked before and already has an answer. If those answers do not fully address your question, please ask a new question.

You could stick

export WINEPREFIX=/home/$USER/.playonlinux/path/to/prefix/

in ~/.bashrc (and then run source ~/.bashrc to load it the first time if you're already in a terminal)

Or just prefix your winetricks commands like:

WINEPREFIX=/home/$USER/.playonlinux/path/to/prefix/ winetricks blah blah blah

I don't use POL but I have in the past and I rather thought there was a button or menu item that let you run various winetricks things without having to run it manually. I'm probably wrong but give it a look.

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