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I was just checking a md5 checksum to see if a file has downloaded correctly or not.

situation is like this. I have downloaded a Linux iso file in Windows and checked the md5 with the Microsoft 'File checksum integrity verifier' utility. it is giving a different checksum from the one displayed on the linux OS website.

Q. IS the md5 checksum different (or same) on different OS types ( winxp, win7, linux etc) ?

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The utility can be different but its function of checking integrity will be same. In your case , The .iso file You have downloaded is faulty or corrupted. You have to download .iso file again.

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No , the md5 sum will be the same on all OS.

See also:

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The Md5 sum is unique. It cannot be changed. The Windows Integrity Checked is not the reliable option. Use Winmd5sum.

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