Like Daniel already explained to you, desktop applications are completely different in the code. In your case, you've used Python to code and Glade to design the window of your application.
Web applications are basically websites that are heavy JavaScript coded.
Python as the programming language can't be used in websites, and your applications GUI is made in Glade, which means it's GTK based XML file. Websites use HTML + CSS to make things visible to you. Lets say their "GUI" is made in the combination of those two, which aren't the same as XML files native desktop applications are using.
You would need to rewrite the code in JavaScript, make a webpage and embed it into a WebKit made with Python and GTK. That's in the case you want it to look a bit like a native app, or use that new Ubuntu WebApp feature that has been released earlier this week.